Measurement

Part:BBa_K1369015:Design

Designed by: Galen Gao   Group: iGEM14_Caltech   (2014-10-17)


fsrB_3xFLAG_N


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 243
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 243
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 502


Design Notes

Inserted 3xFLAG at N-terminus (5' end) of domain in fsrB encoding the prepeptide of the signaling ligand. Constructed by Gibson assembly. Not biobricked.


Source

Enterococcus Faecalis

References